epac2  (Cell Signaling Technology Inc)

Journal: Brazilian journal of otorhinolaryngology

Article Title: cAMP-Epac1 signaling is activated in DDAVP-induced endolymphatic hydrops of guinea pigs.

doi: 10.1016/j.bjorl.2023.03.002

Figure Lengend Snippet: Figure 1 Relative mRNA expression of Epac1, Epac2, Rap1A, Rap1B,

Article Snippet: The primary and secondary antibodies used for western lotting were as follows: rabbit anti-GAPDH primary antiody (KGAA002, 37 kDa, 1:5,000), Horseradish Peroxidase HRP)-conjugated goat anti-mouse IgG secondary antibody KGAA37), and HRP-conjugated goat anti-rabbit IgG secndary antibody (KGAA35) (all Jiangsu KeyGen Biotech), abbit anti-Epac1 primary antibody (GTX41235, Gene Tex, 04 kDa, 1:500), mouse anti-Epac2 primary antibody (SC8326, Santa Cruz Biotechnology, Dallas, TX, 126 kDa, :200), and mouse anti-Rap1 primary antibody (SC-53434, anta Cruz Biotechnology, 44 kDa, 1:200). tatistical analysis he SPSS 20.0 software package (IBM, Armonk, NY) was sed to conduct all statistical analysis.

Techniques: Expressing

Journal: Brazilian journal of otorhinolaryngology

Article Title: cAMP-Epac1 signaling is activated in DDAVP-induced endolymphatic hydrops of guinea pigs.

doi: 10.1016/j.bjorl.2023.03.002

Figure Lengend Snippet: Figure 2 Epac1, Epac2, and Rap1 protein expression in the cochleae of different groups.

Article Snippet: The primary and secondary antibodies used for western lotting were as follows: rabbit anti-GAPDH primary antiody (KGAA002, 37 kDa, 1:5,000), Horseradish Peroxidase HRP)-conjugated goat anti-mouse IgG secondary antibody KGAA37), and HRP-conjugated goat anti-rabbit IgG secndary antibody (KGAA35) (all Jiangsu KeyGen Biotech), abbit anti-Epac1 primary antibody (GTX41235, Gene Tex, 04 kDa, 1:500), mouse anti-Epac2 primary antibody (SC8326, Santa Cruz Biotechnology, Dallas, TX, 126 kDa, :200), and mouse anti-Rap1 primary antibody (SC-53434, anta Cruz Biotechnology, 44 kDa, 1:200). tatistical analysis he SPSS 20.0 software package (IBM, Armonk, NY) was sed to conduct all statistical analysis.

Techniques: Expressing